Coding

Part:BBa_K1202010:Design

Designed by: Furkan Bestepe   Group: iGEM13_ATOMS-TURKIYE   (2013-10-04)


LsrR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 472
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LsrR is inserted to upstream region of Lsr promoter we designed. A constitutive promoter and compatible RBS is attached before the part for coding. Constitutive production of LsrR is planned to repress the Lsr promoter activity. We plan to inhibit this effect by the production of AI-2.


Source

It is originated from E.coli. GeneScript synthesized the sequence we found through our researches.

References